Journal: Journal of Cellular and Molecular Medicine

Article Title: Exogenous pulmonary surfactant prevents the development of intra‐abdominal adhesions in rats

doi: 10.1111/jcmm.12758

Figure Lengend Snippet: Expression and activity of MMP ‐9 in vivo . Gel zymography analysis showed that MMP ‐9 activity was significantly higher in GE ‐S versus GE animals ( P < 0.05) ( A ). In situ zymography analysis of MMP ‐9 expression showed a larger number of cells positive for gelatinase activity in GE ‐S versus GE animals. ( B ) Isolectin B4 ( IB 4) staining of the gastroenterostomy region revealed no significant difference in total number of macrophages ( C , arrows in the left‐hand column) between the GE and GE ‐S animals ( C , first‐ versus second‐row, and D ). The number of ED 1‐positive cells ( E , arrows in the left‐hand column) was significantly higher ( P < 0.001) in GE ‐S versus GE animals ( F ). No significant differences in number of cells double‐labelled for MMP ‐9/ IB 4 were observed between the GE ‐S and GE groups (third column in C ). Note that virtually all cells positive for ED 1 were MMP ‐9+ (third column in E ), scale bar: 200 μm.

Article Snippet: The expression and activity of MMP‐9 were investigated in all rats of groups L, LS, GE and GE‐S by SDS‐PAGE gelatin zymography using a 4% polyacrylamide stacking gel and a 10% polyacrylamide resolving gel (Bio‐Rad Co., Richmond, VA, USA) containing 1.5 mg/ml gelatin (BDH Laboratory Supplies, Poole, UK).

Techniques: Expressing, Activity Assay, In Vivo, Zymography, In Situ, Staining

Journal: PLoS ONE

Article Title: Matrix metalloproteinase promotes elastic fiber degradation in ligamentum flavum degeneration

doi: 10.1371/journal.pone.0200872

Figure Lengend Snippet: A: Representative gelatin zymography analysis of LF fibroblasts in response to IL-6/sIL-6Rα stimulation for 24 h with or without Stattic. B: Quantitative analysis of data shown in panel A (n = 3). C: Concentration of soluble elastin following incubation for 6 h with the supernatant of IL-6-stimulated cultures with or without Stattic (n = 3). Data represent the mean ± SEM. **P < 0.01.

Article Snippet: LF fibroblasts with or without 50 nM Stattic pretreatment were stimulated with IL-6 (300 ng/ml) for 24 h. MMP-2 and -9 activities in the culture supernatant were measured with an SDS-PAGE gelatin zymography kit (Cosmo Bio, Tokyo, Japan) according to the manufacturer’s instructions.

Techniques: Zymography, Concentration Assay, Incubation